Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)), is a truncated peptide derived from the human Myelin Oligodendrocyte Glycoprotein (MOG).

CD8CD3 and CD4CD3 cells were 86% and 94% pure of total CD3 cells after CD8/CD4 bead enrichment, respectively.?These cells were stimulated by MOG35-55 peptide and applied to proliferation assays.?Although the CD8 T cells had a generally lower response to MOG35-55 than CD4 T cells, the response of CD8 T cells was not always dependent on CD4.?CD8 T cell secreted less IFN-γ and IL-4 compared with CD4 T cells[1].

EAE was induced in wildtype B6 na?ve mice by adoptive transfer of MOG35-55-specific T cells from B6 active-induced EAE (aEAE) mice.?A similar EAE score and slight inflammation and demyelination were found in naive B6 mice after transferring of CD8 T cells from immunized B6 mice compared with transfer of CD4 T cells[1].

Female C57BL/6 mice (n?=?20) were induced by myelin oligodendrocyte glycoprotein (MOG)35-55 peptide.?At 14 days after immunization, T cells were isolated from the spleen and purified as CD4 and CD8 T cells by using CD4 and CD8 isolation kits, and then the purity was determined by flow cytometric analysis.?These cells were stimulated by MOG35-55 peptide and applied to proliferation assays.?The interferon-gamma (IFN-γ) and interleukin (IL)-4 secretion of supernatant of cultured CD4 and CD8 T cells were measured by enzyme-linked immunosorbent assays (ELISA).?For adoptive transfer, recipient mice were injected with MOG35-55-specific CD8 or CD4 T cells.?EAE clinical course was measured by EAE score at 0-5 scale and spinal cord was examined by staining with hematoxylin and eosin and Luxol fast blue staining[1].