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ONPG

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Catalog No. T19497Cas No. 369-07-3
Alias 2-Nitrophenyl β-D-galactopyranoside

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.

ONPG

ONPG

🥰Excellent
Purity: 100%
Catalog No. T19497Alias 2-Nitrophenyl β-D-galactopyranosideCas No. 369-07-3
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
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Purity:100%
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Product Introduction

Bioactivity
Description
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
Targets&IC50
β-gal:2.7±0.3 mM(Km), β-gal:464.7±7.8/s(kcat)
In vitro
The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s-1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s-1 and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s-1mM-1) is 4.6-fold higher than that for lactose (37.2 s-1mM-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1].
Alias2-Nitrophenyl β-D-galactopyranoside
Chemical Properties
Molecular Weight301.25
FormulaC12H15NO8
Cas No.369-07-3
SmilesO([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2
Relative Density.1.3709 g/cm3 (Estimated)
Storage & Solubility Information
Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
H2O: 7.4 mg/mL (24.56 mM), Sonication is recommended.
Solution Preparation Table
H2O
1mg5mg10mg50mg
1 mM3.3195 mL16.5975 mL33.1950 mL165.9751 mL
5 mM0.6639 mL3.3195 mL6.6390 mL33.1950 mL
10 mM0.3320 mL1.6598 mL3.3195 mL16.5975 mL
20 mM0.1660 mL0.8299 mL1.6598 mL8.2988 mL

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