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Biotin-LC-LC-NHS

Name: Biotin-LC-LC-NHS
Brand: TargetMol
SKU: T41026
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T41026Cas No. 89889-52-1

Biotin-LC-LC-NHS is a membrane permeable SMCC cross-linker and an NHS ester-activated biotinylation reagent that biotinylates antibodies or other proteins for detection or purification using streptavidin probes or resins.

Biotin-LC-LC-NHS

Catalog No. T41026Cas No. 89889-52-1

Pack Size	Price	Availability
1 mg	$30	In Stock
5 mg	$64	In Stock
10 mg	$93	In Stock
25 mg	$153	In Stock
50 mg	$227	In Stock
100 mg	$337	In Stock
200 mg	$490	In Stock

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COA HNMR

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity

Description	Biotin-LC-LC-NHS is a membrane permeable SMCC cross-linker and an NHS ester-activated biotinylation reagent that biotinylates antibodies or other proteins for detection or purification using streptavidin probes or resins.
In vitro	Biotinylation of the antibody was achieved by reacting Biotin-LC-LC-NHS (1 mg/mL, dissolved in dimethylformamide) with the antibody in the aqueous phase at pH 7.4. The molar ratio of biotin to antibody in the labeling reaction was 10:1, and the final biotin concentration was 70 μM. [1]

Chemical Properties

Molecular Weight	567.7
Formula	C₂₆H₄₁N₅O₇S
Cas No.	89889-52-1

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 100 mg/mL (176.15 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7615 mL	8.8075 mL	17.6149 mL	88.0747 mL
5 mM	0.3523 mL	1.7615 mL	3.5230 mL	17.6149 mL
10 mM	0.1761 mL	0.8807 mL	1.7615 mL	8.8075 mL
20 mM	0.0881 mL	0.4404 mL	0.8807 mL	4.4037 mL
50 mM	0.0352 mL	0.1761 mL	0.3523 mL	1.7615 mL
100 mM	0.0176 mL	0.0881 mL	0.1761 mL	0.8807 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Biotin-LC-LC-NHSBiotinLCLCNHSBiotin LC LC NHS