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CLK1-IN-1

Catalog No. T10836Cas No. 2123491-32-5

CLK1-IN-1 is a potent and selective inhibitor of the Cdc2-like kinase 1 (CLK1; IC50: 2 nM).

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Pack Size	Price	Availability
25 mg	$1,520	6-8 weeks
50 mg	$1,980	6-8 weeks
100 mg	$2,500	6-8 weeks

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Product Introduction

Bioactivity

Description	CLK1-IN-1 is a potent and selective inhibitor of the Cdc2-like kinase 1 (CLK1; IC50: 2 nM).
In vitro	In addition to CLK1, only two kinases have an IC50 value less than 100 nM, namely CLK2 (IC50: 31 nM) and CLK4 (IC50: 8 nM), DYRK1A is the strongest off-target. The effects of CLK1-IN-1 on yellow LC3 puncta also displays obvious dose dependency, and a dose of 10 μM shows the best performance. In addition, in CLK1-IN-1-treated cells, the number of red LC3 puncta (mRFP signals only35) increases compared with that of DMSO-treated cells, indicating the formation of autolysosomes. Importantly, CLK1-IN-1 stimulates the degradation of SQSTM1/p62 and increases the ratio of red LC3 puncta to yellow LC3 puncta, both of which indicate induction of autophagic flux by CLK1-IN-1.
In vivo	APAP exposure results in severe liver injury and treatment with CLK1-IN-1 (i.p., 30 mg/kg) imparts a significant hepatoprotective effect. Treatment with CLK1-IN-1 decreases serum ALT and AST levels significantly such that both marker enzymes return to normal levels.

Chemical Properties

Molecular Weight	409.42
Formula	C24H16FN5O
Cas No.	2123491-32-5

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 25 mg/mL (61.06 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4425 mL	12.2124 mL	24.4248 mL	122.1240 mL
5 mM	0.4885 mL	2.4425 mL	4.8850 mL	24.4248 mL
10 mM	0.2442 mL	1.2212 mL	2.4425 mL	12.2124 mL
20 mM	0.1221 mL	0.6106 mL	1.2212 mL	6.1062 mL
50 mM	0.0488 mL	0.2442 mL	0.4885 mL	2.4425 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc