Columbianetin-beta-D-glucopyranoside exhibits anti-inflammatory and analgesic properties, it also shows strong inhibiting activity against platelet aggregation.

METHODS AND RESULTS: Eight coumarins isolated from the alcohol extract of the Chinese drug 'Duhuo', the root of Angelica pubescents Maxim. f. biserrata Shan et Yuan (Umbelliferae) were elucidated to be columbianetin (I), columbianetin acetate (II), columbiadin (III), osthol (IV), isoimperatorin (V), bergapten (VI), xanthotoxin (VII), and Columbianetin beta-D-glucopyranoside (VIII), by chemical and spectral analysis, compound VIII was isolated from plant for the first time. CONCLUSIONS: All these coumarins were tested on platelet aggregation induced by 2 microns ADP. I, II, III, IV and VIII showed strong inhibiting activity against platelet aggregation.