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F5446

Name: F5446
Brand: TargetMol
SKU: T71890
Rating: 4.5 (1 reviews)

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Catalog No. T71890Cas No. 2304465-89-0

Alias F 5446

F5446 is a selective small molecule SUV39H1 methyltransferase inhibitor. It promotes apoptosis in colorectal cancer cells by inhibiting the deposition of H3K9me3 on the FAS promoter to increase Fas expression, while inhibiting CTL effector gene expression.

F5446

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Purity: 98.56%

Catalog No. T71890Alias F 5446Cas No. 2304465-89-0

Pack Size	Price	Availability
25 mg	$1,520	6-8 weeks
50 mg	$1,980	6-8 weeks
100 mg	$2,500	6-8 weeks

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Purity:98.56%

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COA

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity

Description	F5446 is a selective small molecule SUV39H1 methyltransferase inhibitor. It promotes apoptosis in colorectal cancer cells by inhibiting the deposition of H3K9me3 on the FAS promoter to increase Fas expression, while inhibiting CTL effector gene expression.
In vitro	Methods: SW620 and LS411N cells were treated with F5446 (100, 250 nM, 48 h), then fixed, stained with PI, and analyzed for DNA content by flow cytometry. Results: F5446 treatment induced cell cycle arrest at S phase in both SW620 and LS411N cells in a concentration-dependent manner. Methods: SW620, LS411N and CCD841 cells were treated with F5446 (10, 50, 100, 250, 500, 1000 nM) and analyzed for apoptosis. Results: F5446 induced apoptosis in both tumor cell lines in vitro in a concentration-dependent manner. The normal human colon epithelial cell line CCD841 was less sensitive to F5446 in inducing apoptotic cell death than tumor cells. [1]
In vivo	Methods: F5446 (5, 10 mg/kg, intraperitoneal injection, once every two days, for 26 days) was used to treat SW620 and SW620-5FUR cell-transplanted tumor model mice. Results: F5446 can inhibit tumor growth in vivo in a concentration-dependent manner. [1]
Alias	F 5446

Chemical Properties

Molecular Weight	552.94
Formula	C₂₆H₁₇ClN₂O₈S
Cas No.	2304465-89-0
Smiles	COC(=O)c1cc2-c3c(c[nH]c3C(=O)C(=O)c2n1S(=O)(=O)c1ccc(Cl)cc1)C(=O)OCc1ccccc1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 80 mg/mL (144.68 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8085 mL	9.0426 mL	18.0851 mL	90.4257 mL
5 mM	0.3617 mL	1.8085 mL	3.6170 mL	18.0851 mL
10 mM	0.1809 mL	0.9043 mL	1.8085 mL	9.0426 mL
20 mM	0.0904 mL	0.4521 mL	0.9043 mL	4.5213 mL
50 mM	0.0362 mL	0.1809 mL	0.3617 mL	1.8085 mL
100 mM	0.0181 mL	0.0904 mL	0.1809 mL	0.9043 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

F5446

F5446

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