Kinesore is a KLC2-SKIP Interaction inhibitor.

In kinesore-treated cells, the microtubule network undergoes a complete reorganization, forming loops and bundles, with the lysosomal compartment accumulating into a juxtanuclear position that is sparse in microtubules. At a 50 μM concentration of kinesore, this reorganized nonradial microtubule network phenotype is highly prevalent, occurring in 95±2.4% (n=3, total of 200 cells) of cells. Moreover, in wild-type cells, 50 μM kinesore spurs the transformation of the microtubule network and prompts the emergence of microtubule-rich projections. This alteration is significantly subdued in Kif5B knockout cells, underscoring the necessary role of kinesin-1 in kinesore-induced microtubule remodeling. Titration experiments reveal that this phenotype becomes noticeable at 25 μM kinesore after 1-hour treatment, with minimal effects at concentrations of 12.5 μM or lower. Notably, the radial microtubule array is restored after a 2-hour kinesore washout in cells initially treated for 1 hour, indicating reversibility. This phenomenon of kinesore-induced microtubule network reorganization is consistent across various mammalian normal and cancer cell lines[1].