NP213 TFA is a rapidly acting and first-in-class synthetic antimicrobial peptide (AMP) with anti-fungal activity. It targets the fungal cytoplasmic membrane via membrane perturbation and disruption.

Fungal:

NP213 (500-1000 μg/mL; 18 hours) increases the number of PI-stained T. rubrum NCPF0118 cells in samples [1]. NP213 shows different MIC values against T. rubrum NCPF0118, and the MICs varies depending on the source of the keratin. The MIC values are 16-32 mg/L, 125 mg/L, and 250 mg/L for NP213 in 1640 media containing human nail Keratin, human skin Keratin, and Lamb's wool Keratin, respectively [1].NP213 TFA (2-3 hours; 0-8 μg/ml) has great activity against clinically relevant yeast, including candida spp, Cryptococcus spp, and Trichosporon spp. For all 122 yeast isolates, with the median MIC100 values of 1-2 μg/ml [3].

In Murine models of acute disseminated candidiasis, NP213 is tolerated and efficacious and exhibits a half-life of approximately 4.5 h. NP213 TFA (25 mg/kg) is well tolerated in mice [3].