QWhere can I find purity infomation for future products?
AFor futures products, no purity information or quality inspection can be available. Once the products are available, we will conduct quality inspections to determine the purity and structural information, which will be displayed on our official website.
QWhat should I do if the IC50 obtained for cell killing are significantly different from that presented on the your website?
AThe IC50 you obtain refers to the cell proliferation inhibition assay (half maximal inhibitory concentration), which is different in meaning from the IC50 on our website. The IC50 often refers to the inhibition rate of the target without cell experiments, which is generally determined through kinase or protein purification experiments, and is commonly reported in nanomolar concentrations. However, the IC50 obtained from cell proliferation inhibition assays refers to the half-maximal lethality of the cells, which involves cellular metabolism and penetration, so the concentration is usually higher.
Furthermore, the effect of the same compound on different cell models varies. It is recommended to try increasing the incubation volume and extending the incubation time.
Qwhat frequency should sonication be used?
AIt is recommended to use the conventional ultrasound cleaning frequency
QWhat is the solubility of the product?
AIt is recommended to check the product description and solubility information on our website first. If corresponding information is not available
QCan Tween-80 be used instead of Tween-20?
ATween-20 is not recommended. Tween-80 has better tolerance and has been used as a tool to assess the behavioral effects of experimental drugs and toxins without significant side effects.
Reference: Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.