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Pyronin Y (Pyronine G) is an intercalating cationic dye with specificity for RNA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 mg | $29 | In Stock | |
200 mg | $39 | In Stock | |
1 mL x 10 mM (in DMSO) | $29 | In Stock |
Description | Pyronin Y (Pyronine G) is an intercalating cationic dye with specificity for RNA. |
In vitro | Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting[2]. In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells[3]. |
Alias | Pyronine G, C.I. 45005 |
Molecular Weight | 302.8 |
Formula | C17H19ClN2O |
Cas No. | 92-32-0 |
Smiles | [Cl-].CN(C)c1ccc2cc3ccc(cc3[o+]c2c1)N(C)C |
Relative Density. | no data available |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
Solubility Information | DMSO: 25 mg/mL (82.56 mM), Sonication is recommended. H2O: 4 mg/mL (13.21 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
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