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CD34 Protein, Human, Recombinant (His), Biotinylated

Catalog No. TMPY-05772

CD34 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 29 kDa and the accession number is P28906-1.

CD34 Protein, Human, Recombinant (His), Biotinylated

CD34 Protein, Human, Recombinant (His), Biotinylated

Catalog No. TMPY-05772
CD34 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 29 kDa and the accession number is P28906-1.
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Product Information

Biological Activity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
CD34 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 29 kDa and the accession number is P28906-1.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP28906-1
Synonyms
CD34 molecule
Construction
A DNA sequence encoding the extracellular domain of human CD34 precursor (NP_001020280.1) (Met1-Thr290) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. Predicted N terminal: Ser 32
Protein Purity
> 90 % as determined by SDS-PAGE.
Molecular Weight29 kDa (predicted)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.

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Please read the User Guide of Recombinant Proteins for more specific information.