Testing in progress

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234&P07766.

Human

HEK293 Cells

T3D,CD3d molecule, δ (CD3-TCR complex),CD3d molecule, delta (CD3-TCR complex),CD3-DELTA,CD3-δ,IMD19

The extracellular domain (Met1-Ala105) of Human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; The extracellular domain (Met1-Asp126) of Human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3D/CD3E heterodimer was purified. The purified protein was biotinylated in vitro.

> 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.