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CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234&P07766.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $245 | In Stock | |
100 μg | $589 | 7-10 days | |
200 μg | $1,000 | 7-10 days | |
500 μg | $2,030 | 7-10 days |
Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234&P07766. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-Flag, C-His |
Accession Number | P04234&P07766 |
Synonyms | T3D,IMD19,CD3-δ,CD3-DELTA,CD3d molecule, δ (CD3-TCR complex),CD3d molecule, delta (CD3-TCR complex) |
Construction | A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. The purified protein was biotinylated in vitro. Predicted N terminal: Phe 22 & Asp 23 |
Protein Purity | > 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
Molecular Weight | 31.05 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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