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CD3D & CD3E Heterodimer Protein, Rhesus, Recombinant

Catalog No. TMPY-03328
CD3D & CD3E Heterodimer Protein, Rhesus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.7 kDa and the accession number is G7NCB9.
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Product Information

Biological Activity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
CD3D & CD3E Heterodimer Protein, Rhesus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.7 kDa and the accession number is G7NCB9.
Species
Rhesus
Expression System
HEK293 Cells
TagTag Free
Accession NumberG7NCB9
Synonyms
CD3d molecule, δ (CD3-TCR complex),CD3d molecule, delta (CD3-TCR complex)
Construction
A DNA sequence encoding the rhesus CD3D extracellular domain (F6WI60)(Met1-Ala105) with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rhesus CD3E extracellular domain(XP_014971302.1) (Met1-Asp117) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified. Predicted N terminal: Gln 22 & Phe 22
Protein Purity
> 95 % as determined by SDS-PAGE
Molecular Weight76.7 kDa (predicted); 52 & 43 kDa (reducing conditions)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.

Dose Conversion

You can also refer to dose conversion for different animals. More

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Please read the User Guide of Recombinant Proteins for more specific information.

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