CD40 Ligand Protein, Human, Recombinant (Mammalian)

Catalog No. TMPJ-00150

CD40 Ligand Protein, Human, Recombinant (Mammalian) is expressed in HEK293 mammalian cells. The predicted molecular weight is 16-18 KDa and the accession number is P29965.

CD40 Ligand Protein, Human, Recombinant (Mammalian)

Catalog No. TMPJ-00150

CD40 Ligand Protein, Human, Recombinant (Mammalian) is expressed in HEK293 mammalian cells. The predicted molecular weight is 16-18 KDa and the accession number is P29965.

Pack Size	Price	Availability
10 μg	$116	7-10 days
50 μg	$310	7-10 days
500 μg	$1,900	7-10 days
1 mg	$2,730	7-10 days

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Product Information

Biological Activity	1. Loaded Human CD40-mFc on AMC Biosensor, can bind Human CD40L with an affinity constant of 10.2 nM as determined in BLI assay. (Regularly tested) 2. Loaded Human CD40-Fc on Protein A Biosensor, can bind Human CD40L with an affinity constant of 3.29
Description	CD40 Ligand Protein, Human, Recombinant (Mammalian) is expressed in HEK293 mammalian cells. The predicted molecular weight is 16-18 KDa and the accession number is P29965.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P29965
Synonyms	Tumor Necrosis Factor Ligand Superfamily Member 5,TRAP,TNFSF5,TNF-Related Activation Protein,T-Cell Antigen Gp39,CD40LG,CD40-L,CD40L,CD40 Ligand,CD154
Amino Acid	Met113-Leu261
Construction	Met113-Leu261
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Molecular Weight	16-18 KDa (reducing condition)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.