CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc)

Catalog No. TMPK-00860

CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548.

CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc)

Catalog No. TMPK-00860

Pack Size	Price	Availability
100 μg	$394	7-10 days
500 μg	$1,570	7-10 days
1 mg	$2,640	7-10 days

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Product Information

Biological Activity	Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 25.6ng/ml determined by ELISA.
Description	CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548.
Species	Mouse
Expression System	HEK293 Cells
Tag	N-monomeric hFc
Accession Number	P27548
Synonyms	TRAP,TNFSF5IMD3,TNFSF5,T-BAM,IMD3,IGM,HIGM1,gp39,CD40LIGM,CD40LG,CD40L,CD40 Ligand,CD154
Construction	Met112-Leu260
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	75.6 kDa (predicted). Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 200 mM L-arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.