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Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $228 | In Stock | |
100 μg | $405 | 7-10 days | |
200 μg | $716 | 7-10 days | |
500 μg | $1,520 | 7-10 days |
Biological Information | 1.Measured by its binding ability in a functional ELISA. Immobilized CD40 Protein, Mouse, (ECD, His Tag) at 2 μg/mL (100 μL/well) can bind CD40 Ligand Protein, Human, (hFc Tag), the EC50 of CD40 L-hFc is 70-180 ng/mL.
2.Loaded Recombinant Mouse CD40 Protein, His Tag on His1K Biosensor, can bind Recombinant Mouse CD40 Ligand Protein, hFc Tag with an affinity constant of 87.2nM as determined in BLI assay (Routinely tested). |
Description | CD40 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.81 kDa and the accession number is P27512-1. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | P27512-1 |
Synonyms | Bp50,HIGM1,TRAP,T-BAM,GP39,AI326936,p50,IGM,CD40 molecule, TNF receptor superfamily member 5,Tnfrsf5,IMD3 |
Construction | The Mouse CD40 (NP_035741.2) (Met1-Arg193) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
Molecular Weight | 20.81 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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