Shopping Cart
- Remove All
- Your shopping cart is currently empty
ICAM-1/CD54 Protein, Human, Recombinant (aa 1-480,DDDDK) is expressed in HEK293 mammalian cells. The predicted molecular weight is 50.2 kDa and the accession number is A0A384MEK5.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $451 | In Stock | |
200 μg | $771 | 7-10 days | |
500 μg | $1,560 | 7-10 days |
Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | ICAM-1/CD54 Protein, Human, Recombinant (aa 1-480,DDDDK) is expressed in HEK293 mammalian cells. The predicted molecular weight is 50.2 kDa and the accession number is A0A384MEK5. |
Species | Human |
Expression System | HEK293 Cells |
Tag | Tag Free |
Accession Number | P05362 |
Synonyms | P3.58,intercellular adhesion molecule 1,ICAM-1,CD54,BB2 |
Construction | A DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) with five amino acids (DDDDK) at the C-terminus was expressed and purified. Predicted N terminal: Gln 28 |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
Molecular Weight | 50.2 kDa (predicted); 71.3 kDa (reducing condition, due to glycosylation) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
Copyright © 2015-2024 TargetMol Chemicals Inc. All Rights Reserved.