1. Immobilized Human LILRB1, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. 2. Human LILRB1, hFc Tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 1.49nM as determined in SPR assay.

LILRB1/CD85j/ILT2 Protein, Human, Recombinant (aa 24-458, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 73.8 kDa and the accession number is Q8NHL6-1.

Human

HEK293 Cells

XXbac-BCX85G21.4,MIR7,LIR1,LILRB1,ILT2FLJ37515,ILT-2,ILT2,CD85J

Gly24-His458

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.