Home Tools

DPPH

Catalog No. T11093 CAS 1898-66-4

Synonyms: 2,2-Diphenyl-1-picrylhydrazyl

DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a cell-permeable, stable free radical that is commonly used to evaluate the ability of compounds to act as free radical scavengers or hydrogen donors and to measure the antioxidant activity of tissue extracts

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

DPPH, CAS 1898-66-4

Pack Size	Availability	Price/USD
5 mg	In stock	$ 39.00
10 mg	In stock	$ 46.00
25 mg	In stock	$ 65.00
50 mg	In stock	$ 80.00
100 mg	In stock	$ 97.00
200 mg	In stock	$ 148.00
500 mg	In stock	$ 249.00
1 mL * 10 mM (in DMSO)	In stock	$ 43.00

Bulk Inquiry

Select Batch

Purity: 99.25%

Purity: 96.89%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

DPPH shows a strong absorption band at 517nm. Due to its odd number of electrons, the solution presents a dark purple color, and the absorption disappears as the electron pairs are detached.The resulting decoloration is stoichiometric and depends on the number of electrons absorbed.Alcohol solutions of 0.5 mM are dense in color, and at this concentration, the Lambert-Beer law is followed within the effective range of absorption.DPPH is a fast, simple, inexpensive and widely used method for determining the ability of compounds to act as free radical scavengers or hydrogen donors and for evaluating the antioxidant activity of foods.It can also be used to quantify antioxidants in complex biological systems, solid or liquid samples.The method was simple and easy to determine the total antioxidant capacity and free radical scavenging activity of fruit and vegetable juice.The antioxidant properties of wheat grains and bran, vegetables, conjugated linoleic acid, herbal medicine, edible seed oil and flour in ethanol, acetone, methanol, ethanol and benzene were successfully studied.It is a simple method for the determination of the antioxidant properties of cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxyaromatic compounds in olive oil, fruits, fruit juices and wines.

Synonyms	2,2-Diphenyl-1-picrylhydrazyl
Molecular Weight	394.32
Formula	C18H12N5O6
CAS No.	1898-66-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 24 mg/mL (60.86 mM), Sonication is recommended.

References and Literature

1. Kedare SB, et al. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011 Aug;48(4):412-22.

Citations

1. Qin Z, Zou Y, Zhang Y, et al. Electrospun pullulan nanofiber loading zanthoxylum bungeanum essential oil/β-cyclodextrin inclusion complexes for active packaging. International Journal of Biological Macromolecules. 2022 2. Jia F, Yu B, Li J, et al.Supramolecular Nano‐assembly of Caffeate‐Strengthened Phenylboronic Ester with Multi‐step Ros Scavenging Ability for Targeted Therapy of Acute Kidney Injury.Advanced Healthcare Materials.2301615

Related compound libraries

This product is contained In the following compound libraries：

Ferroptosis Compound Library Antioxidant Compound Library NO PAINS Compound Library Oxidation-Reduction Compound Library Anti-Fibrosis Compound Library Bioactive Compounds Library Max Bioactive Compound Library Anti-Aging Compound Library

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

DPPH 1898-66-4 Others inhibit Inhibitor 2,2-Diphenyl-1-picrylhydrazyl inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.