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S130

Catalog No. T12819 CAS 1160852-22-1

S130 is a high affinity, selectiveATG4B (a major cysteine protease) inhibitor (IC50 of 3.24 μM).It suppresses autophagy flux.

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S130, CAS 1160852-22-1

Pack Size	Availability	Price/USD
5 mg	6-8 weeks	$ 297.00
25 mg	6-8 weeks	$ 987.00
50 mg	6-8 weeks	$ 1,360.00
100 mg	6-8 weeks	$ 1,860.00
1 mL * 10 mM (in DMSO)	6-8 weeks	$ 318.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description	S130 is a high affinity, selectiveATG4B (a major cysteine protease) inhibitor (IC50 of 3.24 μM).It suppresses autophagy flux.
Targets&IC50	ATG4B:3.24 µM
In vitro	S130 suppresses autophagy and activates apoptosis by inhibiting ATG4B, leads to enhanced cytotoxicity. S130 (0-25 μM; 48 hours) induces cell death through inhibiting the activity of ATG4B at a dose higher than 6.3 μM. And such cytotoxicity might not cause cell death through necroptosis. Nutrient deprivation enhances S130-induced cytotoxicity. S130 (0-10μM; 24 hours) suppresses approximately 79% of the cleavage of full-length LC3-GST at the 10 μM, while no substrates were processed in ATG4B KO cells. S130 displays obvious inhibitory effects on ATG4B.
In vivo	Tumor growth suppressed by S130 (20 mg/kg; i.p.; daily; 3 weeks), S130 shows an efficient in vivo antitumor effect with a sound safety on vital organs.

Molecular Weight	387.47
Formula	C24H25N3O2
CAS No.	1160852-22-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 125 mg/mL (322.61 mM), Sonication is recommended.

References and Literature

1. Fu Y, et al. Discovery of a small molecule targeting autophagy via ATG4B inhibition and cell death of colorectal cancer cells in vitro and in vivo. Autophagy. 2018 Sep 20:1-17.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

S130 1160852-22-1 Proteases/Proteasome Cysteine Protease S-130 S 130 inhibitor inhibit

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