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PMSF

Catalog No. T0789Cas No. 329-98-6
Alias Phenylmethylsulfonyl fluoride, Benzylsulfonyl fluoride

Phenylmethanesulfonyl fluoride (PMSF (Phenylmethylsulfonyl fluoride)) is an enzyme inhibitor that inactivates IRC-50 arvin, subtilisin, and the fatty acid synthetase complex.

PMSF

PMSF

Purity: 99.9%
Catalog No. T0789Alias Phenylmethylsulfonyl fluoride, Benzylsulfonyl fluorideCas No. 329-98-6
Phenylmethanesulfonyl fluoride (PMSF (Phenylmethylsulfonyl fluoride)) is an enzyme inhibitor that inactivates IRC-50 arvin, subtilisin, and the fatty acid synthetase complex.
Pack SizePriceAvailabilityQuantity
50 mg$33In Stock
100 mg$46In Stock
200 mg$48In Stock
500 mg$55In Stock
1 mL x 10 mM (in DMSO)$50In Stock
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Purity:99.9%
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Product Introduction

Bioactivity
Description
Phenylmethanesulfonyl fluoride (PMSF (Phenylmethylsulfonyl fluoride)) is an enzyme inhibitor that inactivates IRC-50 arvin, subtilisin, and the fatty acid synthetase complex.
In vitro
Treatment with PMSF (intraperitoneal injection) in mice elicits cannabinoid-like effects, providing analgesia (ED50: 86 mg/kg), hypothermia (ED50: 224 mg/kg), and catalepsy (ED50: 206 mg/kg). When administered to Sprague-Dawley rats, PMSF induces a dose-dependent analgesic effect and significantly potentiates the analgesic effect of β-endorphin in vivo. By inhibiting fatty acid amide hydrolase (FAAH) activity, PMSF suppresses typical cannabinoid or Δ(9)-tetrahydrocannabinol-like effects in ICR mice. Pretreatment with 30 mg/kg PMSF before the injection of [3H]-labeled cannabinoids results in a notable increase in brain cannabinoid levels after 5 minutes compared to [3H]-THC. PMSF pretreatment at 30 mg/kg enhances the cannabinoid-induced effects on the tail-flick response (analgesic effect), locomotion, and spontaneous activity by 5, 8, and 10-fold respectively. Administering PMSF 12 hours before paraoxon (PSP) protects hens from delayed neurotoxicity, whereas administration 4 hours later exacerbates the neurotoxic effects. PMSF pretreatment also prevents organophosphate-induced delayed neuropathy in hens and inhibits neuropathilament degeneration induced by tri-ortho-tolyl phosphate.
In vivo
PMSF is a specific inhibitor of phospholipase C involved in the hydrolysis of phosphatidylinositol. In the longitudinal smooth muscle of guinea pig ileum, PMSF (2 mM) almost completely inhibits carbachol-activated synthesis of phosphatidylinositol muscarinic, without affecting synthesis activated by potassium. PMSF transiently inhibits muscle contractions induced by both potassium and carbachol. As an acetylcholinesterase inhibitor, PMSF outperforms BSF (at 8 times the concentration of PMSF), with BSF being sixfold less effective. PMSF rapidly inhibits the activity of trypsin purified from the human pancreas and acetylcholinesterase in human erythrocytes but has a negligible effect on human trypsin. In Trypanosoma brucei, PMSF inhibits the addition of ethanolamine phosphate to the intermediate of glycosylphosphatidylinositol and the acylation of the glycosyl residue in the blood GPI intermediates as well as the addition of ethanolamine phosphate and the acylation of glycosyl in the procyclic form, with no inhibition observed on the latter in mammalian HeLa cells.
AliasPhenylmethylsulfonyl fluoride, Benzylsulfonyl fluoride
Chemical Properties
Molecular Weight174.19
FormulaC7H7FO2S
Cas No.329-98-6
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Ethanol: 17.4 mg/mL (100 mM)
DMSO: 55 mg/mL (315.75 mM)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM5.7409 mL28.7043 mL57.4086 mL287.0429 mL
5 mM1.1482 mL5.7409 mL11.4817 mL57.4086 mL
10 mM0.5741 mL2.8704 mL5.7409 mL28.7043 mL
20 mM0.2870 mL1.4352 mL2.8704 mL14.3521 mL
50 mM0.1148 mL0.5741 mL1.1482 mL5.7409 mL
100 mM0.0574 mL0.2870 mL0.5741 mL2.8704 mL

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