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Necrostatin 2

Catalog No. T12203LCas No. 852391-19-6

Necrostatin 2 is an effective necroptosis inhibitor. Necrostatin 2 is also a RIPK1 inhibitor. EC50 for inhibition of necroptosis in FADD-deficient Jurkat T cells treated with TNF-α is 0.05 μM.

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Pack Size	Price	Availability
2 mg	$78	5 days
5 mg	$166	5 days
1 mL x 10 mM (in DMSO)	$148	5 days

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Product Introduction

Bioactivity

Description

Necrostatin 2 is an effective necroptosis inhibitor. Necrostatin 2 is also a RIPK1 inhibitor. EC50 for inhibition of necroptosis in FADD-deficient Jurkat T cells treated with TNF-α is 0.05 μM.

In vitro

Necrostatin 2 demonstrates efficacy in preventing cell death via necroptosis across various cellular systems[1], including full protection of L929 cells from TNF-α-induced necroptosis at a concentration of 30 μM. It also counteracts necrosis in L929 cells caused by both TNF-α and the pan-caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (zVAD.fmk)[2]. Furthermore, in studies with FADD-deficient Jurkat T cells exposed to TNF-α, Necrostatin 2 reliably halts necroptosis, evidenced by an EC50 of 50 nM, indicating robust and specific activity at a concentration as low as 0.05 μM[3].

Chemical Properties

Molecular Weight	277.71
Formula	C13H12ClN3O2
Cas No.	852391-19-6

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 100 mg/mL (360.09 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.6009 mL	18.0044 mL	36.0088 mL	180.0439 mL
5 mM	0.7202 mL	3.6009 mL	7.2018 mL	36.0088 mL
10 mM	0.3601 mL	1.8004 mL	3.6009 mL	18.0044 mL
20 mM	0.1800 mL	0.9002 mL	1.8004 mL	9.0022 mL
50 mM	0.0720 mL	0.3601 mL	0.7202 mL	3.6009 mL
100 mM	0.0360 mL	0.1800 mL	0.3601 mL	1.8004 mL

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In Vivo Formulation Calculator (Clear solution)

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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

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mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

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Tech Support

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