Necrostatin-34

Necrostatin-34 (2-{[3-cyano-4-(4-methylphenyl)-6-oxo-1,4) is a RIPK1 kinase inhibitor, stabilizing RIPK1 kinase in an inactive conformation by occupying a distinct binding pocket in the kinase domain.

Catalog No. T9095Cas No. 375835-43-1

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Purity:98.80%

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COA LCMS HNMR

Necrostatin-34

Catalog No. T9095Cas No. 375835-43-1

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Pack Size	Price	Availability
1 mg	$48	In Stock
5 mg	$118	In Stock
10 mg	$173	In Stock
25 mg	$322	In Stock
50 mg	$465	In Stock
100 mg	$648	In Stock
200 mg	$875	In Stock
1 mL x 10 mM (in DMSO)	$137	In Stock

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Product Introduction

Bioactivity

Description	Necrostatin-34 (2-{[3-cyano-4-(4-methylphenyl)-6-oxo-1,4) is a RIPK1 kinase inhibitor, stabilizing RIPK1 kinase in an inactive conformation by occupying a distinct binding pocket in the kinase domain.
In vitro	Necrostatin-34 (10?μM) inhibits the dimerization-induced RIPK1 activation as examined by phosphorylation of Ser166 (p-S166) of RIPK1, a biomarker for RIPK1 activation. Necrostatin-34 may block TNFα-induced complex II formation by inhibiting the activation of RIPK1 kinase. Necrostatin-34 shows IC50 values of 667 nM and 134 nM for TNFα in FADD def-Jurkat cells and L939 cells, respectively.
Alias	2-{[3-cyano-4-(4-methylphenyl)-6-oxo-1,4

Chemical Properties

Molecular Weight	384.48
Formula	C₁₈H₁₆N₄O₂S₂
Cas No.	375835-43-1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 125 mg/mL (325.11 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6009 mL	13.0046 mL	26.0092 mL	130.0458 mL
5 mM	0.5202 mL	2.6009 mL	5.2018 mL	26.0092 mL
10 mM	0.2601 mL	1.3005 mL	2.6009 mL	13.0046 mL
20 mM	0.1300 mL	0.6502 mL	1.3005 mL	6.5023 mL
50 mM	0.0520 mL	0.2601 mL	0.5202 mL	2.6009 mL
100 mM	0.0260 mL	0.1300 mL	0.2601 mL	1.3005 mL

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

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