S1R Agonist 1 (Compound 6b) hydrochloride is a selective S1R agonist, displaying K i values of 0.93 nM for S1R and 72 nM for S2R, and has demonstrated neuroprotective effects against ROS and NMDA-induced neurotoxicity [1].

S1R agonist 1 (Compound 6b; 0.1-5 μM) hydrochloride significantly enhances neurite outgrowth induced by nerve growth factor (NGF) in a dose-dependent manner in PC12 cells and, at 1 μM for 24 hours, inhibits Rotenone-induced damage in SHSY5Y cells [1]. Additionally, it demonstrates neuroprotective effects against NMDA stimulation in SHSY5Y cells at 0.1-5 μM over 24 hours and shows no cytotoxicity in A549, LoVo, and Panc-1 cells at 0-10 μM over 24-72 hours [1].

Compound 6b, a selective S1R agonist at concentrations ranging from 0.1 to 50 μM, was administered over 120 hours, resulting in the death of 4 out of 8 zebrafish embryos at a 10 μM concentration [1].