B7-1 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00178

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. B7-1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.8 kDa and the accession number is P33681-1.

B7-1 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00178

Pack Size	Price	Availability
100 μg	329 €	7-10 days
500 μg	1.330 €	7-10 days
1 mg	2.213 €	7-10 days

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Product Information

Biological Activity	1. Immobilized Human B7-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 0.12μg/ml determined by ELISA. 2. Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay.
Description	Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. B7-1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.8 kDa and the accession number is P33681-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P33681-1
Synonyms	LAB7,CD80 molecule,CD80,CD28LG1,BB1,B7-1,B71,B7.1,B7
Construction	Val35-Asn242
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	26.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.