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Costunolide

Costunolide
Costunolide (Costus lactone) has anti-inflammatory and anti-oxidant properties and mediates apoptosis.
Catalog No. T2902Cas No. 553-21-9
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Purity:100%
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Costunolide

Catalog No. T2902Cas No. 553-21-9
Costunolide (Costus lactone) has anti-inflammatory and anti-oxidant properties and mediates apoptosis.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
5 mg$55In Stock
10 mg$79In Stock
25 mg$128In Stock
50 mg$192In Stock
100 mg$285In Stock
500 mg$686In Stock
1 mL x 10 mM (in DMSO)$50In Stock
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Product Introduction

Bioactivity
Description
Costunolide (Costus lactone) has anti-inflammatory and anti-oxidant properties and mediates apoptosis.
Targets&IC50
Telomerase (MCF-7 cells):90 μM, Telomerase (MDA-MB-231 cells):65 μM, FPTase:20 μM
In vitro
Costunolide reduces neovascularization induced by ethyl ester resin scale plaster in the corneal micropocket model of mice. It inhibits angiogenesis by blocking the vascular endothelial growth factor signaling pathway.
In vivo
Costunolide has been shown to inhibit cell proliferation in a dose-dependent manner over a 48-hour treatment in various human tumor cells, including A549, SK-OV-3, SK-MEL-2, XF498, and HCT-15. It induces apoptosis in HL-60 human leukemia cells through ROS-mediated mitochondrial permeability transition and cytochrome C release. Additionally, Costunolide exhibits significant antifungal activity against organisms such as Trichophyton rubrum, T. mentagrophytes, and Microsporum gypseum. The compound also suppresses telomerase activity and cell proliferation in MCF-7 and MDA-MB-231 cells in a concentration and time-dependent manner. Furthermore, Costunolide dose-dependently inhibits the farnesylation of lamin B by farnesyl protein transferase, demonstrating its diverse biochemical impacts.
Kinase Assay
Telomerase activity assay: The telomerase activity is measured by the TRAP assay using the TRAPez Telomerase Detection Kit, which includes primers of a 36-bp internal control (IC) for quantifying the amplification of telomerase activity within a linear range close to 2.5 logs. For RNase treatment, 10μL of extract are incubated with 1μg of RNase at 37 °C for 20 minutes. The products of the TRAP assay are resolved by electrophoresis in a nondenaturing12% PAGE in a buffer containing 0.5 × Tris–borate EDTA and detected by autoradiograph. For quantification of TRAP products, the dried gels are exposed to Fuji Imaging Plate at room temperature. Results are corrected for background, and a standard value of 100 is given to the untreated control cell signal. Signal intensities of Costunolide-treated cells are compared to the standard and are expressed as a fraction of the maximum value of 100. [1]
Cell Research
1) Plate 500-10,000 cells in 200 μL media per well in a 96 well plate. Leave 8 wells empty for blank controls. 2) Incubate (37 °C, 5% CO2) overnight to allow the cells to attach to the wells. 3) Add 2 μL of Costunolide dissolved in DMSO to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the samples into the media. 5) Incubate (37 °C, 5% CO2) for 48 hours to allow Costunolide to take effect. 6) Make 2 mL or more of MTT solution per 96 well plate at 5 mg/mL in PBS. Do not make a stock as MTT in solution is not stable long-term. 7) Add 20 μL MTT solution to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the MTT into the media. 8) Incubate (37 °C, 5% CO2) for 1-5 hours to allow the MTT to be metabolized. 9) Dump off the media. (Dry plate on paper towels to remove residue if necessary. 10) Resuspend formazan (MTT metabolic product) in 200 μL DMSO. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the formazan into the solvent. 11) Read optical density at 560 nm and subtract background at 670 nm. Optical density should be directly correlated with cell quantity. (Only for Reference)
AliasCostus lactone, NSC 106404, Costunolid, (+)-Costunolide
Chemical Properties
Molecular Weight232.32
FormulaC15H20O2
Cas No.553-21-9
Storage & Solubility Information
Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 45 mg/mL (193.7 mM)
Ethanol: 11.6 mg/mL (50 mM)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM4.3044 mL21.5220 mL43.0441 mL215.2204 mL
5 mM0.8609 mL4.3044 mL8.6088 mL43.0441 mL
10 mM0.4304 mL2.1522 mL4.3044 mL21.5220 mL
20 mM0.2152 mL1.0761 mL2.1522 mL10.7610 mL
50 mM0.0861 mL0.4304 mL0.8609 mL4.3044 mL
DMSO
1mg5mg10mg50mg
100 mM0.0430 mL0.2152 mL0.4304 mL2.1522 mL

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