TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA.

METHODS: Human hepatocellular carcinoma cells HepG2 were treated with TCEP hydrochloride (100-400 µM) for 3 days, and cell viability was detected by MTT assay.
RESULTS: The viability of HepG2 was significantly reduced to 25.68% and 70.92% after treatment with 200 and 400 µM TCEP for 3 days, whereas the lowest concentration of TCEP (100 µM) did not significantly reduce the viability of HepG2 (3.44%). [1]
METHODS: Human peripheral blood mononuclear cell PBMCs were treated with TCEP hydrochloride (0.5-1 mM) for 24 h. Morphological and necrotic changes were assessed by Hoechst 33342/PI staining.
RESULTS: Necrosis was observed in cells treated with 0.5 mM of TCEP. [2]

METHODS: To investigate the neurotoxicity and its mechanism in mice, TCEP hydrochloride (10-100 mg/kg) was administered by gavage to Kunming mice once a day for 30 days.
RESULTS: Compared with the control group, the high dose TCEP group showed a significant decrease in water intake and a significant increase in liver and spleen organ indices. In the water maze test, the escape latency of TCEP-exposed mice was longer than that of the control group, and the total swimming course of the high-dose TCEP group was higher than that of the control group, and the swimming time of the target quadrant was significantly shorter.TCEP exposure may cause neurotoxicity by increasing thyroid hormones and inducing oxidative damage in mice. [3]