Broussochalcone A is a natural antioxidant and xanthine oxidase inhibitor (IC50 = 2.21 μM) that scavenges free radicals, inhibits iron-induced lipid peroxidation, and reduces nitric oxide synthesis in LPS-activated macrophages. Broussochalcone A induces apoptosis in human renal carcinoma cells by increasing ROS levels and activating the FOXO3 signaling pathway. Broussochalcone A is also a novel NR4A1 inhibitor that induces apoptosis in pancreatic cancer cells through NR4A1-dependent pathways.

Methods: Broussochalcone A (1-30 μM) was incubated with DPPH (100 μM) at room temperature for 30 min, and the decrease in absorbance at 517 nm was measured. The DPPH scavenging activity of BCA was investigated.
Results: The scavenging activity of Broussochalcone A was concentration-dependent.
Methods: Cells were treated with Broussochalcone A (1-20 μM) for 30 min and then stimulated with LPS (1 mg/mL). After 24 h, nitrite levels in the culture medium were analyzed by Griess reaction. Its effect on nitrite production in LPS-activated RAW 264.7 macrophages was investigated.
Results: Broussochalcone A inhibited nitrite production in a concentration-dependent manner, and its IC50 value was calculated to be 11.3±0.8 μM. [2]