Galanin Receptor Ligand M35 TFA is a high-affinity ligand and antagonist of the galanin receptor (Kd = 0.1 nM).

Galanin Receptor Ligand M35 TFA exhibits dual effects on galanin-mediated inhibition of Forskolin-stimulated cyclic AMP production in Rin m 5F cells. At low concentrations (1 nM), co-applied with galanin (10 nM), it reverses galanin's inhibitory effect, while at higher concentrations (15 and 30 nM), it functions as a galanin receptor agonist, inhibiting cAMP production. These effects are not additive, reflecting its mixed agonist/antagonist properties due to its chimeric nature acting solely at galanin receptors[1].

Galanin Receptor Ligand M35 TFA (6 nM/10 μL; i.c.v.) enhances the performance of male Sprague-Dawley rats in the swim maze task by shortening escape latency, reducing failure rates in reaching the platform, and decreasing path length to the hidden platform[3].