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Product Introduction

Bioactivity

Description	Rhodamine B (Brilliant Pink B) is used as a tracer dye in water to determine the rate and direction of flow and transport.
Alias	Tetraethylrhodamine, Rhodamine B, Rhodamine O, Brilliant Pink B, Basic Violet 1

Chemical Properties

Molecular Weight	479.01
Formula	C₂₈H₃₁ClN₂O₃
Cas No.	81-88-9
Smiles	[Cl-].CCN(CC)c1ccc2c(-c3ccccc3C(O)=O)c3ccc(cc3oc2c1)=[N+](CC)CC
Relative Density.	0.79 g/cm3 at 20℃

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 55 mg/mL (114.82 mM), Heating is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0876 mL	10.4382 mL	20.8764 mL	104.3820 mL
5 mM	0.4175 mL	2.0876 mL	4.1753 mL	20.8764 mL
10 mM	0.2088 mL	1.0438 mL	2.0876 mL	10.4382 mL
20 mM	0.1044 mL	0.5219 mL	1.0438 mL	5.2191 mL
50 mM	0.0418 mL	0.2088 mL	0.4175 mL	2.0876 mL
100 mM	0.0209 mL	0.1044 mL	0.2088 mL	1.0438 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Rhodamine BInhibitorinhibitBasic Violet-10Basic Violet10Basic Violet 10