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ANI-7

Catalog No. T10325 CAS 931417-26-4

ANI-7 is an activator of aryl hydrocarbon receptor (AhR) pathway. ANI-7 inhibits the growth of multiple cancer cells and potently and selectively inhibits the growth of MCF-7 breast cancer cells (GI50: 0.56 μM).

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ANI-7, CAS 931417-26-4

Pack Size	Availability	Price/USD
5 mg	In stock	$ 64.00
10 mg	In stock	$ 126.00
25 mg	In stock	$ 229.00
50 mg	In stock	$ 383.00
100 mg	In stock	$ 573.00
500 mg	In stock	$ 1,250.00
1 mL * 10 mM (in DMSO)	In stock	$ 39.00

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Purity: 99.21%

Biological Description

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Description

In vitro

ANI-7 (2.5 μM; 24 hours; MCF10A and MDA-MB-468 cells) treatment induces significant S-phase and G2 + M-phase cell cycle arrest within 24 hours of treatment in MDA-MB-468 cells. ANI-7 (2 μM; 12-24 hours; MDA-MB-468 cells) treatment results in a significant increase in the content and phosphorylation of CHK2, and induces a significant increase in H2AXɣ in MDA-MB-468 cells, indicative of DNA double-strand damage. Comparisons of the GI50 values show that ANI-7 produces a GI50 value of 0.38 μM in MCF-7 cells, whereas values of 3.0-42 μM are observed in cell lines from lung, colon, ovary, neuronal, glial, prostate, and pancreas. The only other tumor type that shows appreciable growth inhibition by ANI-7 is the A431 vulva cell line (GI50 of 0.51μM). ANI-7 potently inhibits the growth of T47D, ZR-75-1, MCF-7, SKBR3, and MDA-MB-468 breast cancer cells (GI50 range of 0.16-0.38 μM), moderately inhibits the growth of BT20 and BT474 cells (GI50 range of 1-2 μM), and essentially fails to inhibit the growth of MDA-MB-231 and MCF10A cells (GI50 range of 17-26 μM). Moreover, ANI-7 maintained its ability to inhibit the growth of drug-resistant cells (MCF-7/VP16: GI50 of 0.21 μM) [1].

Molecular Weight	263.12
Formula	C13H8Cl2N2
CAS No.	931417-26-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 18.7 mg/mL (71.3 mM), Sonification is recommended.

References and Literature

1. Gilbert J, et al. (Z)-2-(3,4-Dichlorophenyl)-3-(1H-Pyrrol-2-yl)Acrylonitrile Exhibits Selective Antitumor Activity in Breast Cancer Cell Lines via the Aryl Hydrocarbon Receptor Pathway. Mol Pharmacol. 2018 Feb;93(2):168-177. 2. Baker JR, et al. Dichlorophenylacrylonitriles as AhR Ligands That Display Selective Breast Cancer Cytotoxicity in vitro. ChemMedChem. 2018 Jul 18;13(14):1447-1458. 3. Mark Tarleton, et al. Library synthesis and cytotoxicity of a family of 2-phenylacrylonitriles and discovery of an estrogen dependent breast cancer lead compound. Medicinal Chemistry Communication. January 20112. (1):31-37.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Covalent Inhibitor Library Bioactive Compounds Library Max Bioactive Compound Library Kinase Inhibitor Library Anti-Cancer Compound Library

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LY2880070 CCT241533 AZD-7762 M443 CCT244747 CHK1-IN-3 Tuvusertib SAR-020106

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

ANI-7 931417-26-4 Cell Cycle/Checkpoint Chk ANI 7 ANI7 inhibitor inhibit

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