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Daidzein

Catalog No. T1238 CAS 486-66-8

Synonyms: Isoflavone

Daidzein (Isoflavone) is an isoflavone extract from soy, which is an inactive analog of the tyrosine kinase inhibitor genistein. It has antioxidant and phytoestrogenic properties.

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Daidzein, CAS 486-66-8

Pack Size	Availability	Price/USD
500 mg	In stock	$ 45.00
1 g	In stock	$ 57.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 98.84%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Daidzein (Isoflavone) is an isoflavone extract from soy, which is an inactive analog of the tyrosine kinase inhibitor genistein. It has antioxidant and phytoestrogenic properties.
In vivo	Daidzein results in reduced body weight, a fact that may be explained by reduced feed consumption in female rats. Daidzein results in slight, but not significant, decreases in ovarian and uterine weights, and mammary gland size in female rats. [5]
Cell Research	Daidzein is dissolved in DMSO and then diluted with appropriate media[1]. HEK293T cells are plated on 24-well plates at a cell density of approximately 2.5×104 cells/well and are grown to 70-80% confluence. Cells are then transiently transfected with a PPAR-α or PPAR-γ expression plasmid, and a plasmid containing the luciferase gene under the control of three tandem PPAR response elements (PPRE × 3 TK-luciferase) using an X-treme GENE HP DNA Transfection Reagent. Renilla luciferase control vectors are co-transfected to control for transfection efficiency. After transfection, cells are cultured for another 24 h in medium containing DMSO or various concentrations (6.25, 12.5, 25 μM) of Daidzein. Cells are lysed, and luciferase activity is measured and expressed as fold induction, that is normalized to the activity of the renilla luciferase control plasmid[1].
Source	Natural Products > Fabaceae > Glycine

Synonyms	Isoflavone
Molecular Weight	254.24
Formula	C15H10O4
CAS No.	486-66-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 25.4 mg/mL (100 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Hämäläinen M, et al. Mediators Inflamm. 2007;22007:45673. 2. Sugimoto E, et al. Biochem Pharmacol,2000, 59(5), 471-475. 3. Jia TL, et al. Biochem Pharmacol,2003, 65(5), 709-715. 4. Gao YH, et al. Mol Cell Biochem, 1999, 194(1-2), 93-97. 5. Lamartiniere CA, et al. Toxicol Sci,2002, 65(2), 228-238. 6. Kim E, et al. Daidzein Augments Cholesterol Homeostasis via ApoE to Promote Functional Recovery in Chronic Stroke. J Neurosci. 2015 Nov 11;35(45):15113-26. 7. Sakamoto Y1, et al. The Dietary Isoflavone Daidzein Reduces Expression of Pro-Inflammatory Genes through PPARα/γ and JNK Pathways in Adipocyte and Macrophage Co-Cultures. PLoS One. 2016 Feb 22;11(2):e0149676.

Citations

1. Ferreira R S B, Farias F O, de Araujo E J S, et al.Deep eutectic solvents as an alternative for extraction of flavonoids from soybean (Glycine max (L) Merrill) and okara: An experimental and computational approach based on COSMO-SAC model.Food Research International.2023: 113266. 2. Rashid F, Ghimire S, Mangalam A K, et al.A UPLC-MS/MS Based Rapid, Sensitive, and Non-Enzymatic Methodology for Quantitation of Dietary Isoflavones in Biological Fluids.Molecules.2023, 28(18): 6729.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Approved Drug Library Traditional Chinese Medicine Monomer Library Drug Repurposing Compound Library Tobacco Monomer Library Inhibitor Library Anti-Cancer Drug Library Anti-Cancer Compound Library Ferroptosis Compound Library Lipid Metabolism Compound Library Flavonoid Natural Product Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Daidzein 486-66-8 DNA Damage/DNA Repair Metabolism PPAR Endogenous Metabolite inhibit Inhibitor Isoflavone Peroxisome proliferator-activated receptors inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.