Hirsutanonol is a secondary metabolite from the bark of Alnus glutinosa. Hirsutanonol has potent antioxidant and free radical scavenging activities and exhibits an inhibition effect on mitochondrial lipid peroxidation. Hirsutanonol can be used for studies

Hirsutanonol shows significant inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cyclooxygenase-2 (COX-2) expression in immortalized human breast epithelial MCF10A cells. Hirustenone and hirsutanonol show promising anti-filarial activity both in vitro and in vivo studies.Hirsutanonol shows potent cytotoxic activities against murine B16 melanoma cells and human SNU-C1 gastric cancer cells, it also has a chemoprotective effect on human lymphocyte DNA. Hirsutanonol (1 µg/mL) decreased the frequency of micronuclei by 63.6 %, exerting a much stronger effect than the synthetic protector amifostine (17.2 %, 1 µg/mL)[1].