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Isopteropodine

Catalog No. TN1790 CAS 5171-37-9

Synonyms: Uncarine E

Isopteropodine (Uncarine E) is an isoyohimbine-type octindole alkaloid isolated from Hamelia patens micropropagated and has antimicrobial activity and stimulates the immune system. Isopteropodine has antimicrobial activity and stimulates the immune system.Isopteropodine is a positive modulator of muscarinic M1 and 5-HT2 receptors and a PXR activator that induces contraction of the rat myometrium.

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Isopteropodine, CAS 5171-37-9

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Description

In vitro

Here, we investigated for the first time the antiproliferative and apoptotic effects of highly purified oxindole alkaloids, namely Isopteropodine (A1), pteropodine (A2), isomitraphylline (A3), uncarine F (A4) and mitraphylline (A5) obtained from Uncaria tomentosa, a South American Rubiaceae, on human lymphoblastic leukaemia T cells (CCRF-CEM-C7H2). Four of the five tested alkaloids inhibited proliferation of acute lymphoblastic leukaemia cells. Furthermore, the antiproliferative effect of the most potent alkaloids pteropodine (A2) and uncarine F (A4) correlated with induction of apoptosis. After 48 h, 100 micromol/l A2 or A4 increased apoptotic cells by 57%. CEM-C7H2 sublines with tetracycline-regulated expression of bcl-2, p16ink4A or constitutively expressing the cowpox virus protein crm-A were used for further studies of the apoptosis-inducing properties of these alkaloids. Neither overexpression of bcl-2 or crm-A nor cell-cycle arrest in G0/G1 phase by tetracycline-regulated expression of p16INK4A could prevent alkaloid-induced apoptosis[1]

Synonyms	Uncarine E
Molecular Weight	368.43
Formula	C21H24N2O4
CAS No.	5171-37-9

Storage

store at low temperature,keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 36.84 mg/mL (100 mM), Sonification is recommended.

References and Literature

1. Oxindole alkaloids from Uncaria tomentosa induce apoptosis in proliferating, G0/G1-arrested and bcl-2-expressing acute lymphoblastic leukaemia cells.Br J Haematol. 2006 Mar;132(5):615-22. 2. Kang TH, et al. Pteropodine and isopteropodine positively modulate the function of rat muscarinic M(1) and 5-HT(2) receptors expressed in Xenopus oocyte. Eur J Pharmacol. 2002 May 24;444(1-2):39-45.

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Keywords

Isopteropodine 5171-37-9 GPCR/G Protein Microbiology/Virology Neuroscience Antibacterial AChR 5-HT Receptor Uncarine E inhibitor inhibit

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