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UNC 0631

Catalog No. T2354 CAS 1320288-19-4

Synonyms: UNC0631

UNC 0631 is a effectvie histone methyltransferase G9a inhibitor (IC50=4 nM).

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UNC 0631, CAS 1320288-19-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 47.00
2 mg	In stock	$ 68.00
5 mg	In stock	$ 112.00
10 mg	In stock	$ 197.00
25 mg	In stock	$ 420.00
50 mg	In stock	$ 618.00
100 mg	In stock	$ 863.00
1 mL * 10 mM (in DMSO)	In stock	$ 158.00

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Purity: 98.76%

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Description	UNC 0631 is a effectvie histone methyltransferase G9a inhibitor (IC50=4 nM).
Targets&IC50	G9a:4 nM
Kinase Assay	SAHH-coupled assays: This assay utilizes SAHH to hydrolyze the methyltransfer product SAH to homocysteine and adenosine in the presence of adenosine deaminase which converts adenosine to inosine. The homocysteine concentration is then determined through conjugation of its free sulfhydryl moiety to a thiol-sensitive fluorophore, ThioGlo. For IC50 determinations, assay mixtures are prepared in 25 mM potassium phosphate buffer pH 7.5, 1 mM EDTA, 2 mM MgCl2, 0.01% Triton X-100 with 5 μM SAHH, 0.3 U/mL of adenosine deaminase, 25 μM SAM, and 15 μM ThioGlo. G9a, GLP, SETD7, SETD8, PRMT3 and SUV39H2 are assayed at 25 nM, 100 nM, 200 nM, 250 nM, 500 nM and 100 nM, respectively. Inhibitors are added at concentrations ranging from 4 nM to 16 μM. After 2 min incubation, reactions are initiated by addition of the histone peptides: 10 μM H3(1–25) for G9a, 20 μM H3(1–25) for GLP, 100 μM H3(1–25) for SETD7, 500 μM H4(1–24) for SETD8, 10 μM H4(1–24) for PRMT3 and 200 μM H3K9Me1 (1–15) for SUV39H2. The methylation reaction is followed by monitoring the increase in fluorescence using Biotek Synergy2 plate reader with 360/40 nm excitation filter and 528/20 nm emission filter for 20 min in 384 well-plate format. Activity values are corrected by subtracting background caused by the peptide or the protein. IC50 values are calculated using Sigmaplot. Standard deviations are calculated from two independent experiments.
Cell Research	MDA-MB-231, PC3, HCT116 cells are cultured in RPMI with 10% FBS, 22RV1 cells in alphaMEM and 10% FBS, MCF7 and IMR90 cells in DMEM with 10% FBS. Cells are treated with inhibitors for 48 h. The media is removed and replaced with DMEM 10% FBS without phenol red supplemented with 1 mg/mL of MTT and incubated for 1–2 h. Live cells reduce yellow MTT to purple formazan. The resulting formazanis solubilized in acidified isopropanol and 1% Triton. Formazan signal absorbance is measured at 570 nm and corrected for the 650 nm background. IC50s are calculated using GraphPad Prizm statistical package with sigmoidal variable slope dose response curve fit.(Only for Reference)

Synonyms	UNC0631
Molecular Weight	635.93
Formula	C37H61N7O2
CAS No.	1320288-19-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 5 mg/mL (7.86 mM), Heating is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 100 mg/mL (157.25 mM), Heating is recommended.

References and Literature

1. Liu F, et al. J Med Chem. 2011, 54(17), 6139-6150.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compound Library Histone Modification Compound Library Epigenetics Compound Library Chromatin Modification Compound Library Bioactive Compounds Library Max

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Keywords

UNC 0631 1320288-19-4 Chromatin/Epigenetic Histone Methyltransferase H3K9me2 p53 UNC-0631 chromatin inhibit EHMT1 G9a KMT1D cell-penetration PKMT Inhibitor UNC0631 inhibitor

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