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Trypsin

🥰Excellent
Catalog No. TN6872Cas No. 9002-07-7

Trypsin is a serine protease that can be isolated from fish to hydrolyze lysine or arginine carboxy-side proteins. With the anti-inflammatory activity, Trypsin could induce the cell membrane fusion of PDCoV-infected cells through the interaction between the S glycoprotein of PDCoV and pAPN, activate PAR2 and PAR4, and promote cell proliferation and differentiation. Trypsin can be used to promote wound healing and study neurogenic inflammation.

Trypsin

Trypsin

🥰Excellent
Catalog No. TN6872Cas No. 9002-07-7
Trypsin is a serine protease that can be isolated from fish to hydrolyze lysine or arginine carboxy-side proteins. With the anti-inflammatory activity, Trypsin could induce the cell membrane fusion of PDCoV-infected cells through the interaction between the S glycoprotein of PDCoV and pAPN, activate PAR2 and PAR4, and promote cell proliferation and differentiation. Trypsin can be used to promote wound healing and study neurogenic inflammation.
Pack SizePriceAvailabilityQuantity
100 mg$39In Stock
500 mg$89In Stock
1 g$133In Stock
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Potency:≥250 U/mg
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Product Introduction

Bioactivity
Description
Trypsin is a serine protease that can be isolated from fish to hydrolyze lysine or arginine carboxy-side proteins. With the anti-inflammatory activity, Trypsin could induce the cell membrane fusion of PDCoV-infected cells through the interaction between the S glycoprotein of PDCoV and pAPN, activate PAR2 and PAR4, and promote cell proliferation and differentiation. Trypsin can be used to promote wound healing and study neurogenic inflammation.
In vitro
Trypsin (10, 50 ng/mL; 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells.[2]
Trypsin (0.05%; 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium.[3]
Trypsin (20 -150 ng/mL; 5 days) potentiates PBMC differentiation.[4]
In vivo
Trypsin, when administered intradermally at doses of 100 to 500 μg per site in 50 μL of saline, elicits scratching behavior in mice.[5]
Chemical Properties
Cas No.9002-07-7
Smiles[#6]-[#6](-[#8])=O.[#6]-c1cc(-[#7]-[#6](=O)-[#6@H](-[#6]-[#6]-[#6]\[#7]=[#6](\[#7])-[#7])-[#7]-[#6](=O)-[#6@H](-[#6]-[#8])-[#7]-[#6](=O)-[#6@H](-[#6]-c2ccccc2)-[#7]-[#6](=O)-[#8]C([#6])([#6])[#6])cc2oc(=O)ccc12
Relative Density.1.37 g/cm3 at 20℃
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 90.0 mg/mL, Sonication and heating to 60℃ are recommended.

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TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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