1.Loaded Biotinylated Human FcγRIIIB / CD16b (NA1) recombinant protein (His & Avi Tag) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 4.3 μM as determined in BLI assay(QC tested). 2. The purity of CD16b Protein, Human, Recombinant (NA1 allotype, His & AVI Tag) is more than 90% and the molecular weight of this protein is around 26-44 kDa verified by SEC-MALS.

CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.75 kDa and the accession number is AAA35881.1.

Human

HEK293 Cells

Fc fragment of IgG receptor IIIb,CD16,FCRIII,Fc gamma RIIIb,FCGR3,FCRIIIb,Fc γ RIIIb,FCR-10,FCG3,CD16b

The Human FCGR3B (AAA35881.1) (Met1-Ser200) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.