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CD16b Protein, Human, Recombinant (NA1s, His)

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CD16b Protein, Human, Recombinant (NA1s, His)

Catalog No. TMPY-05097

CD16b Protein, Human, Recombinant (NA1s, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is AAA35881.1.

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Pack Size	Price	Availability	Quantity
100 μg	$600	7-10 days
1 mg	$3,910	7-10 days

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Biological Description

Biological Information	1.Loaded Human FcγRIIIB / CD16b (NA1) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.99 μM as determined in a BLI assay. 2.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRIIIB / CD16b (NA1) recombinant protein with an affinity constant of 0.16 μM as determined in a BLI assay. 3.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRIIIB / CD16b (NA1) recombinant protein with an affinity constant of 0.16 μM as determined in a BLI assay. 4.Captured IgG1 on protein A Chip can bind Human FcγRIIIB / CD16b (NA1) recombinant protein with an affinity constant of 0.22 μM as determined in an SPR assay (Biacore T200). 5. The purity of Human CD16B / FCGR3B Protein (His Tag, NA1 allotypes) is more than 95% and the molecular weight of this protein is around 27-39 kDa verified by SEC-MALS.
Description	CD16b Protein, Human, Recombinant (NA1s, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is AAA35881.1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	AAA35881.1
Synonyms	Fc γ RIIIb,CD16,Fc fragment of IgG receptor IIIb,Fc gamma RIIIb,FCRIII,FCGR3,FCG3,CD16b,FCRIIIb,FCR-10
Construction	The Human FCGR3B (AAA35881.1) (Met1-Ser200) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC
Molecular Weight	22.4 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.