Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

ATN-224

ATN-224
ATN-224 (Bis(choline)tetrathiomolybdate) inhibits SOD1 activity in endothelial cells, an effect that is dose dependent with an IC50 of 17.5±3.7 nM. ATN-224 is an oral Cu2+/Zn2+-superoxide dismutase 1 (SOD1) inhibitor.
Catalog No. T14341Cas No. 649749-10-0
Select Batch
Purity:99.45%
Contact us for more batch information

Resource Download

ATN-224

Purity: 99.45%
Catalog No. T14341Alias Bis(choline)tetrathiomolybdateCas No. 649749-10-0

ATN-224 (Bis(choline)tetrathiomolybdate) inhibits SOD1 activity in endothelial cells, an effect that is dose dependent with an IC50 of 17.5±3.7 nM. ATN-224 is an oral Cu2+/Zn2+-superoxide dismutase 1 (SOD1) inhibitor.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
1 mg$30In Stock
5 mg$64In Stock
10 mg$109In Stock
25 mg$218In Stock
50 mg$372In Stock
100 mg$596In Stock
1 mL x 10 mM (in DMSO)$71In Stock
Bulk & Custom
Add to Cart
Questions
View More

Related Compound Libraries of "ATN-224"

Product Introduction

Bioactivity
Description
ATN-224 (Bis(choline)tetrathiomolybdate) inhibits SOD1 activity in endothelial cells, an effect that is dose dependent with an IC50 of 17.5±3.7 nM. ATN-224 is an oral Cu2+/Zn2+-superoxide dismutase 1 (SOD1) inhibitor.
Targets&IC50
SOD1 (endothelial cells):17.5±3.7 nM
In vitro
ATN-224 is an orally-available inorganic small molecule that inhibits the copper/zinc-dependent enzyme, superoxide dismutase 1 (Cu/Zn-SOD1), in endothelial and tumor cells[2] amd it is able to inhibit SOD1 activity in endothelial cells, an effect that is dose dependent with an IC50 of 17.5±3.7 nM. ATN-224 inhibits FGF-2-induced ERK1/2 phosphorylation in a dose-dependent and time-dependent manner with an IC50 between 1.25 and 2.5 μM, consistent with the IC50 for the inhibition of proliferation[1], and it inhibits the proliferation of both HUVEC (IC50=1.4±0.3 μM; n=5) and it is also able to inhibit the activity of purified bovine SOD1 with an IC50 of 0.33±0.03 μM after 24 hours of incubation. ATN-224 has a specific and high affinity for copper ions (108 mol/L-1) and shows no binding to calcium, iron, magnesium, zinc, or manganese ions at concentrations up to 1 mM as determined by isothermal titration calorimetry. The SOD1 inhibition by ATN-224 is time dependent, reaching maximal inhibition at ~16 hours. ATN-224 seems to inhibit SOD1 by depleting the enzyme of copper.
In vivo
Oral administration of ATN-224 leads to the inhibition of angiogenesis before any measurable reduction in copper levels in either plasma or the Matrigel plug is observed. Moreover, ATN-224 significantly (P<0.05) suppresses angiogenesis in the Matrigel plug model in mice, regardless of whether it is directly added to the plug or administered orally. These findings indicate that ATN-224's anti-angiogenic effects are not dependent on the depletion of copper[1].
AliasBis(choline)tetrathiomolybdate
Chemical Properties
Molecular Weight432.56
FormulaC10H28MoN2O2S4
Cas No.649749-10-0
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 50 mg/mL (115.59 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.3118 mL11.5591 mL23.1182 mL115.5909 mL
5 mM0.4624 mL2.3118 mL4.6236 mL23.1182 mL
10 mM0.2312 mL1.1559 mL2.3118 mL11.5591 mL
20 mM0.1156 mL0.5780 mL1.1559 mL5.7795 mL
50 mM0.0462 mL0.2312 mL0.4624 mL2.3118 mL
100 mM0.0231 mL0.1156 mL0.2312 mL1.1559 mL

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
%Tween 80
%ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy ATN-224 | purchase ATN-224 | ATN-224 cost | order ATN-224 | ATN-224 chemical structure | ATN-224 in vivo | ATN-224 in vitro | ATN-224 formula | ATN-224 molecular weight