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Pack Size | Price | Availability | Quantity |
---|---|---|---|
1 mg | $47 | In Stock | |
5 mg | $147 | In Stock | |
10 mg | $277 | In Stock | |
25 mg | $469 | In Stock | |
50 mg | $675 | In Stock | |
100 mg | $942 | In Stock | |
1 mL x 10 mM (in DMSO) | $258 | In Stock |
Description | Ac-DEVD-AFC is a fluorogenic substrate |
In vitro | Caspase activation was measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-amino-4-trifluoromethyl coumarin (Ac-DEVD-AFC). We show that this substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-amino-4-trifluoromethyl coumarin (AFC). Following cell disruption, released AFC was separated on HPLC and detected by fluorescence. The appearance of AFC in cells was blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases were responsible for the cleavage. Caspase activity was first noted after about 2 h of incubation with doxorubicin or dactinomycin, the production of AFC being linear with time afterward[1]. |
Molecular Weight | 729.61 |
Formula | C30H34F3N5O13 |
Cas No. | 201608-14-2 |
Storage | keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year | ||||||||||
Solubility Information | DMSO: 40 mg/mL (54.82 mM) H2O: insoluble | ||||||||||
Solution Preparation Table | |||||||||||
DMSO
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