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Arachidonic acid

Arachidonic acid
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Purity:99.78%
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Arachidonic acid

Catalog No. T4129Cas No. 506-32-1
Arachidonic acid (Immunocytophyte) is an unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
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Pack SizePriceAvailabilityQuantity
5 mg$39In Stock
10 mg$55In Stock
25 mg$77In Stock
50 mg$97In Stock
100 mg$122In Stock
200 mg$178In Stock
500 mg$297In Stock
1 mL x 10 mM (in DMSO)$43In Stock
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Product Introduction

Bioactivity
Description
Arachidonic acid (Immunocytophyte) is an unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
In vitro
METHODS: RAW264.7 and PBMC-derived macrophages were treated with Arachidonic acid (40-80 µM) for 12-24 h and cell viability was determined by CCK8 assay.
RESULTS: Arachidonic acid significantly inhibited macrophage viability in a dose-dependent manner within 12 h, and more inhibition was observed between 12 h and 24 h. Arachidonic acid was also shown to inhibit macrophage viability in a dose-dependent manner. [1]
METHODS: Human breast cancer cells, MDA-MB-231, were treated with Arachidonic acid (8 µM) for 48 h. Caspase activity was measured using a spectrofluorophotometer.
RESULTS: MDA-MB-231 cells stimulated with Arachidonic acid for 48 h showed a significant increase in caspase-3 activity, and Arachidonic acid also induced significant activation of caspase-8 and caspase-9. [2]
In vivo
METHODS: To test the effect on the inflammatory response, Arachidonic acid (150 mg/kg in 1% CMC Na) was administered orally to C57BL/6 mice with high-fat diet (HFD)-induced cardiac injury every two days for eight weeks.
RESULTS: Arachidonic acid treatment prevented MD2/TLR4 dimerization, induction of inflammatory factors, and tissue damage through TLR4-mediated inflammatory responses in a high-fat diet obese mouse model. [3]
AliasImmunocytophyte, arachidonate, Immunocytophyt, Vevodar
Chemical Properties
Molecular Weight304.47
FormulaC20H32O2
Cas No.506-32-1
Storage & Solubility Information
Storagekeep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
5% DMSO+95% Saline: 0.6 mg/mL (1.97 mM, precipitation)
DMSO: 100 mg/mL (328.4 mM)
Solution Preparation Table
DMSO/5% DMSO+95% Saline
1mg5mg10mg50mg
1 mM3.2844 mL16.4220 mL32.8440 mL164.2198 mL
DMSO
1mg5mg10mg50mg
5 mM0.6569 mL3.2844 mL6.5688 mL32.8440 mL
10 mM0.3284 mL1.6422 mL3.2844 mL16.4220 mL
20 mM0.1642 mL0.8211 mL1.6422 mL8.2110 mL
50 mM0.0657 mL0.3284 mL0.6569 mL3.2844 mL
100 mM0.0328 mL0.1642 mL0.3284 mL1.6422 mL

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