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Dihydrocytochalasin B

Name: Dihydrocytochalasin B
Brand: TargetMol
SKU: T11042
Rating: 4.5 (1 reviews)

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Catalog No. T11042Cas No. 39156-67-7

Alias Cytochalasin H2B

Dihydrocytochalasin B (H2CB) is a cell division inhibitor that prevents cytokinesis, alters cell morphology, disrupts actin structures in Swiss/3T3 mouse fibroblasts, and inhibits DNA synthesis induced by serum growth factors in quiescent cultures. Low doses of H2CB lead to cell rounding and the loss of actin microfilament bundles.

Dihydrocytochalasin B

😃Good

Catalog No. T11042Alias Cytochalasin H2BCas No. 39156-67-7

Pack Size	Price	Availability
500 μg	$195	35 days
1 mg	$368	35 days
5 mg	$1,450	35 days

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COA

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity

Description	Dihydrocytochalasin B (H2CB) is a cell division inhibitor that prevents cytokinesis, alters cell morphology, disrupts actin structures in Swiss/3T3 mouse fibroblasts, and inhibits DNA synthesis induced by serum growth factors in quiescent cultures. Low doses of H2CB lead to cell rounding and the loss of actin microfilament bundles.
Targets&IC50	P388/ADR cells:28 μM
In vitro	Dihydrocytochalasin B (2-10 X 10 (-7) M) disrupts the actin structure of Swiss/3T3 mouse fibroblasts and inhibits the ability of serum growth factor to stimulate DNA synthesis in static cultures. Low doses of H2CB cause cell rounding and loss of actin microfilaments, but they do not interfere with glucose or thymidine transport. [1] Dihydrocytochalasin B enhances reexpression of the phenotype of transformed growth factor-β induced differentiated chondrocytes without stimulating collagen synthesis. [2] Dihydrocytochalasin B (25 μg/mL) inhibited active calcium transport in intestinal absorption cells and led to increased Ca concentration in mucosal scrape. [3] The IC50 and IC80 of P388/ADR leukemia cell lines inhibited by Dihydrocytochalasin B were 28 μM and 48 μM, respectively. [4]
In vivo	Dihydrocytochalasin B (10 μM) is not toxic to zebrafish. Dihydrocytochalasin B is 20 times less toxic in mice than cytorelaxin D. [5]
Alias	Cytochalasin H2B

Chemical Properties

Molecular Weight	481.62
Formula	C₂₉H₃₉NO₅
Cas No.	39156-67-7
Smiles	O=C1[C@]23[C@]([C@H](CC4=CC=CC=C4)N1)([C@H](C)C(=C)[C@@H](O)[C@@]2(/C=C/C[C@H](C)CCC[C@@H](O)CCC(=O)O3)[H])[H]
Relative Density.	1.194g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 20 mg/mL (41.53 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0763 mL	10.3816 mL	20.7633 mL	103.8163 mL
5 mM	0.4153 mL	2.0763 mL	4.1527 mL	20.7633 mL
10 mM	0.2076 mL	1.0382 mL	2.0763 mL	10.3816 mL
20 mM	0.1038 mL	0.5191 mL	1.0382 mL	5.1908 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

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A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

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