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MMAE-d8

MMAE-d8
D8-MMAE (D8-Monomethyl auristatin E) is an effective mitotic inhibitor and tubulin inhibitor, deuterated MMAE.
Catalog No. T10948Cas No. 2070009-72-0

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MMAE-d8

Catalog No. T10948Alias D8-Monomethyl auristatin E, D8-MMAECas No. 2070009-72-0

D8-MMAE (D8-Monomethyl auristatin E) is an effective mitotic inhibitor and tubulin inhibitor, deuterated MMAE.
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1 mg$1,0907-10 days
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Product Introduction

Bioactivity
Description
D8-MMAE (D8-Monomethyl auristatin E) is an effective mitotic inhibitor and tubulin inhibitor, deuterated MMAE.
In vitro
Antibody-drug conjugates (ADC) contain targeted antibodies with effective small molecule payloads. The ADC is produced against different receptors on the anaplastic large cell lymphoma L-82 line, but delivers the same cytotoxic payload (monomethyl auristatin E, MMAE). ADC-mediated cytotoxicity is not related to target expression or drug: antibody ratio. LC-MS was used to measure the concentration of MMAE in the L-82 tumor parallel cohort by the same treatment protocol. Although there was no difference in tumor volume between the treatment groups 3 days after administration, intratumoral MMAE measurements showed two patterns. First, the MMAE concentration in the tumor increases in proportion to the ADC dose, which corresponds to stronger antitumor activity. Second, intra-tumor MMAE concentrations obtained after treatment with cOKT9-vcMMAE and cAC10-vcMMAE were similar at each dose, which is consistent with the observation that tumors responded similarly to these two ADCs.
In vivo
In tumor xenograft models, the MMAE concentration in the tumor is always correlated with the degree of tumor growth inhibition. IHC analysis showed that tumors that were not combined with control treatment consisted of CD30 + and CD30 cells, presumably because they did not kill CD30 + or CD30- Karpas 299 cells. Only CD30-cells were found in tumors treated with cAC10-vcMMAF, indicating that cAC10-vcMMAF eliminated most CD30 + cells. Interestingly, at the end of the study, two tumors recurring from the treatment of cAC10-vcMMAE were also found to be CD30-, indicating that in the remaining two tumors, a small portion of CD30-cells may have escaped bystanders Kill.
AliasD8-Monomethyl auristatin E, D8-MMAE
Chemical Properties
Molecular Weight726.03
FormulaC39H59D8N5O7
Cas No.2070009-72-0
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 100 mg/mL (137.74 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.3774 mL6.8868 mL13.7735 mL68.8677 mL
5 mM0.2755 mL1.3774 mL2.7547 mL13.7735 mL
10 mM0.1377 mL0.6887 mL1.3774 mL6.8868 mL
20 mM0.0689 mL0.3443 mL0.6887 mL3.4434 mL
50 mM0.0275 mL0.1377 mL0.2755 mL1.3774 mL
100 mM0.0138 mL0.0689 mL0.1377 mL0.6887 mL

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