QHow long does sonication usually take?
AThe sonication time depends on the amount and concentration of the substance
QWhat do "mg/mL" and "mM" mean?
Amg/mL" and "mM" are two different units of measurement, but represent the same maximum solubility. You can convert between them using the molar concentration calculator on our website.
QCan the inhibitor DMSO stock solution be directly diluted with buffer to create a gradient?
AIn most cases, it can dissolve. However, sometimes organic reagents may precipitate when directly added to an aqueous medium. It is recommended to first dilute the inhibitor with DMSO to form a gradient, and then add the diluted inhibitor to the buffer or cell culture medium. Some inhibitors may only dissolve in the aqueous phase at their working concentrations.
For example, if a final concentration of 1 μM is desired in cell experiments, the 10 mM DMSO stock solution can be diluted to 1 mM with DMSO, and then 2 μL can be drawn and added to 2 mL of saline/PBS/cell culture medium, resulting in a final concentration of 1 μM.
To avoid precipitation of the drug, it is recommended to preheat the mother liquor and culture medium to 37°C before dilution to avoid serious precipitation caused by low temperature. If precipitation occurs during the dilution process, it is suggested to use ultrasonic heating to redissolve the compound.
QHow to store inhibitor products?
ASolid-form products can be stored at -20°C for more than 3 years.
Stock solutions are typically stored at -80°C and can be stored for more than 1 year. It is recommended to aliquot the product to avoid repeated freeze-thaw cycles.
For short-term storage, samples can be kept at 4°C for more than one week.
QWhere can I find purity infomation for future products?
AFor futures products, no purity information or quality inspection can be available. Once the products are available, we will conduct quality inspections to determine the purity and structural information, which will be displayed on our official website.