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5-Ethynyluridine

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Purity:100%

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COA HNMR HPLC

5-Ethynyluridine

Catalog No. T36971Cas No. 69075-42-9

5-Ethynyluridine can be used to label newly synthesized RNA. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs.

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Pack Size	Price	Availability
5 mg	$47	In Stock
10 mg	$80	In Stock
25 mg	$148	In Stock
50 mg	$263	In Stock
100 mg	$382	In Stock
200 mg	$573	In Stock
1 mL x 10 mM (in DMSO)	$52	In Stock

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Related Compound Libraries of "5-Ethynyluridine"

Product Introduction

Bioactivity

Description

5-Ethynyluridine is a clickable form of uridine.1It is incorporated into cellular RNA, but not DNA, and has been used to detect transcriptionin vitroandin vivo.
1.Jao, C.Y., and Salic, A.Exploring RNA transcription and turnover in vivo by using click chemistryProc. Natl. Acad. Sci. USA105(41)15779-15784(2008)

In vivo

EU labelling can be used to examine transcription in vivo in mouse models of nervous system disorders. Injection of EU directly into the cerebellum results in reproducible labelling of newly transcribed RNA in cerebellar neurons and glia, with cell type-specific differences in relative labelling intensities, such as Purkinje cells exhibiting the highest levels. EU-labelling accumulating into cytoplasmic inclusions, indicating that EU, like other modified uridines, may introduce non-physiological properties in labelled RNAs[1].

Chemical Properties

Molecular Weight	268.22
Formula	C11H12N2O6
Cas No.	69075-42-9

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMF: 10 mg/mL

PBS (pH 7.2): 5 mg/mL

DMSO: 55 mg/mL (205.06 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7283 mL	18.6414 mL	37.2828 mL	186.4141 mL
5 mM	0.7457 mL	3.7283 mL	7.4566 mL	37.2828 mL
10 mM	0.3728 mL	1.8641 mL	3.7283 mL	18.6414 mL
20 mM	0.1864 mL	0.9321 mL	1.8641 mL	9.3207 mL
50 mM	0.0746 mL	0.3728 mL	0.7457 mL	3.7283 mL
100 mM	0.0373 mL	0.1864 mL	0.3728 mL	1.8641 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc