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Product Introduction

Bioactivity

Description	Orotic acid (Vitamin B13) is an intermediate product in pyrimidine metabolism.
In vitro	Orotic acid increases cell proliferation and decreases apoptosis in serum-starved SK-Hep1 hepatocellular carcinoma cells, which may ascribe to the inhibition of AMP-activated protein kinase (AMPK) phosphorylation and thus activation of mammalian target of rapamycin complex 1 (mTORC1) [1].
In vivo	Male Fischer 344 rats (130-150 g) to two-thirds PH in the absence or in the presence of Orotic acid (a 300-mg tablet of Orotic acid methyl ester implanted intraperitoneally at the time of two-thirds PH). treatment with Orotic acid resulted in a near-100% inhibition of RNR induced by two-thirds PH in rat liver, as monitored by enzyme activity and protein level [2]. Feeding 1% Orotic acid with diet decreased the phosphorylation of AMPK and increased the maturation of SREBP-1 and the expression of SREBP-responsive genes in the rat liver. Orotic acid-induced lipid accumulation was also completely inhibited by rapamycin. Mouse hepatocytes and mice were resistant to Orotic acid-induced lipogenesis because of little if any response in AMPK and downstream effectors [3].
Alias	Vitamin B13, 6-Carboxyuracil

Chemical Properties

Molecular Weight	156.1
Formula	C₅H₄N₂O₄
Cas No.	65-86-1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 55 mg/mL (352.34 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.4061 mL	32.0307 mL	64.0615 mL	320.3075 mL
5 mM	1.2812 mL	6.4061 mL	12.8123 mL	64.0615 mL
10 mM	0.6406 mL	3.2031 mL	6.4061 mL	32.0307 mL
20 mM	0.3203 mL	1.6015 mL	3.2031 mL	16.0154 mL
50 mM	0.1281 mL	0.6406 mL	1.2812 mL	6.4061 mL
100 mM	0.0641 mL	0.3203 mL	0.6406 mL	3.2031 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

inhibitInhibitorpyrimidinenucleotidesVitamin B-13RNAOrotic acidhepatomegalyEndogenous MetaboliteNucleoside Antimetabolite/AnalogsteatosisVitamin B 13ureahepatic