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GSK2200150A

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Catalog No. T4531Cas No. 1443138-53-1

GSK2200150A is an anti-tuberculosis (TB) agent identified by high-throughput screening (HTS) campaign.

GSK2200150A

GSK2200150A

🥰Excellent
Purity: 99.24%
Catalog No. T4531Cas No. 1443138-53-1
GSK2200150A is an anti-tuberculosis (TB) agent identified by high-throughput screening (HTS) campaign.
Pack SizePriceAvailabilityQuantity
5 mg$39In Stock
10 mg$64In Stock
25 mg$122In Stock
50 mg$197In Stock
100 mg$293In Stock
200 mg$437In Stock
1 mL x 10 mM (in DMSO)$43In Stock
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Purity:99.24%
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Product Introduction

Bioactivity
Description
GSK2200150A is an anti-tuberculosis (TB) agent identified by high-throughput screening (HTS) campaign.
Targets&IC50
M. tuberculosis (strain H37Rv):0.38 μM (MIC)
In vitro
GSK2200150A, a novel agent with a spirocycle core, exhibits strong antimycobacterial properties against the virulent Mycobacterium tuberculosis strain (H37Rv), demonstrating a minimal inhibitory concentration (MIC) of 0.38 μM [1].
Cell Research
GSK2200150A is tested for activity at either single concentration (100 μM) or serially diluted in 10 μL of purified H2O in triplicate in 96 well microtiter plates. M. tuberculosis H37Rv is grown in complete Middlebrook 7H9 media containing albumin, dextrose and catalase (ADC), 20% Tween 80 and 50% glycerol. A bacterial suspension (90 μL) at OD600 nm of 0.001 is added to the wells and incubated for 7 days. Resazurin (10 μL; 0.05%(w/v)) is then added, incubated for 24 h at 37°C, and fluorescence measured at 590 nm using a FLUOstar Omega microplate reader. After subtraction of background fluorescence from all wells, the percentage mycobacterial survival is determined by comparing the fluorescence of wells containing compounds compared to control wells not treated with compound[1].
Chemical Properties
Molecular Weight357.47
FormulaC20H23NO3S
Cas No.1443138-53-1
SmilesC(N1CCC2(CC1)OCCc1sccc21)c1ccc2OCCOc2c1
Relative Density.1.31 g/cm3 (Predicted)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 45 mg/mL (125.88 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.7974 mL13.9872 mL27.9744 mL139.8719 mL
5 mM0.5595 mL2.7974 mL5.5949 mL27.9744 mL
10 mM0.2797 mL1.3987 mL2.7974 mL13.9872 mL
20 mM0.1399 mL0.6994 mL1.3987 mL6.9936 mL
50 mM0.0559 mL0.2797 mL0.5595 mL2.7974 mL
100 mM0.0280 mL0.1399 mL0.2797 mL1.3987 mL

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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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