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Product Introduction

Bioactivity

Description	Urethane (Ethylurethane) was an antineoplastic agent .Now is used for other medicinal purposes.
Targets&IC50	AMPA receptor:34 mM(EC50), Glycine receptor:46 mM(EC50), GABAA receptor:64 mM(EC50), α4β2 nAChR:114 mM(EC50), NMDAR:70 mM(EC50)
In vitro	urethane has a spectrum of action on ion channels, which is distinct from other anesthetics. It significantly potentiates the current responses of both GABAA and glycine receptors in a reversible and concentration-dependent manner. Conversely, urethane (10–300 mM) inhibits the responses of NMDA and AMPA receptors. Also, urethane potentiates the function of an nACh receptor and neuronal nicotinic acetylcholine, γ-aminobutyric acid A, and glycine receptors[3].
In vivo	Urethane, a carcinogenic substance, is favored for acute in vivo electrophysiological experiments because it induces long-lasting steady level of anesthesia with muscle relaxation and minimally affects the autonomic and cardiovascular systems[2]. Urethane affects both inhibitory and excitatory systems but the magnitude of the alterations is less than that produced by other more selective anesthetics[3]. But also, Urethane anesthesia is usually restricted to terminal (acute) experiments due to its potential long-term toxicity[1].
Alias	Ethylurethane, Ethyl carbamate, Carbamic acid ethyl ester

Chemical Properties

Molecular Weight	89.09
Formula	C₃H₇NO₂
Cas No.	51-79-6

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 55 mg/mL (617.35 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	11.2246 mL	56.1230 mL	112.2460 mL	561.2302 mL
5 mM	2.2449 mL	11.2246 mL	22.4492 mL	112.2460 mL
10 mM	1.1225 mL	5.6123 mL	11.2246 mL	56.1230 mL
20 mM	0.5612 mL	2.8062 mL	5.6123 mL	28.0615 mL
50 mM	0.2245 mL	1.1225 mL	2.2449 mL	11.2246 mL
100 mM	0.1122 mL	0.5612 mL	1.1225 mL	5.6123 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

inhibitcarbamicParasitenarcosismutagenacidInhibitorBacterialPesticideclastogenUrethane