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7α,25-Dihydroxycholesterol

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7α,25-Dihydroxycholesterol

Catalog No. T14063Cas No. 64907-22-8

7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells[1][2], and it is highly potent at activating EBI2 (EC50=140 pM; Kd=450 pM).

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Pack Size	Price	Availability
1 mg	$143	35 days
5 mg	$655	35 days
10 mg	$1,230	35 days

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Product Introduction

Bioactivity

Description	7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells[1][
In vitro	7α, 25-dihydroxycholesterol (7α,25-OHC) stimulates the migration of EBI2-expressing mouse B and T cells with half-maximum effective concentration values around 500 pM in vitro. However, it had no effect on EBI2-deficient cells[1].
In vivo	In vivo, B cells lacking EBI2 or normal B cells rendered insensitive through pre-treatment with 7α,25-Dihydroxycholesterol (1 μM; 1.5 hours) display decreased migration to spleen follicular regions[1].
Alias	7α,25-OHC

Chemical Properties

Molecular Weight	418.65
Formula	C27H46O3
Cas No.	64907-22-8

Storage & Solubility Information

Storage

store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 1 mg/mL (2.39 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3886 mL	11.9432 mL	23.8863 mL	119.4315 mL

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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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7α,25-Dihydroxycholesterol