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Acetylcysteine

Catalog No. T0875   CAS 616-91-1
Synonyms: Reactive Oxygen Species, NAC, LNAC, Ferroptosis, Acetylcysteine, Influenza Virus, Inhibitor, Endogenous Metabolite, inhibit, N-Acetylcysteine, Apoptosis, N-Acetyl-L-cysteine

Acetylcysteine is the N-acetyl derivative of CYSTEINE. It is used as a mucolytic agent to reduce the viscosity of mucous secretions. It has also been shown to have antiviral effects in patients with HIV due to inhibition of viral stimulation by reactive oxygen intermediates.

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Acetylcysteine, CAS 616-91-1
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Purity: 98%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Acetylcysteine is the N-acetyl derivative of CYSTEINE. It is used as a mucolytic agent to reduce the viscosity of mucous secretions. It has also been shown to have antiviral effects in patients with HIV due to inhibition of viral stimulation by reactive oxygen intermediates.
In vitro N-acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. N-acetylcysteine also prevents the death of PC12 cells and sympathetic neurons[2]. N-acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. N-acetylcysteine protects neuronal cells from death evoked by the withdrawal of trophic support. N-acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. N-acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it is suggested that N-acetylcysteine interferes with redox-sensitive steps in the NGF mechanism[3].
In vivo N-acetylcysteine (150, 300 mg/kg) treatment significantly reduces liver transaminases in all groups of treatment, mostly in group N-acetylcysteine 300. Lung glutathione peroxidase is significantly increased in group N-acetylcysteine 300 (P=0.04), while the other oxidation biomarkers show no significant differences[1].
Cell Research For survival experiments, washed cells are resuspended in RPM1 1640 medium and plated in 0.5 mL at a density of 8-10×105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but to avoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. For experiments involving "primed" PC12 cells, cultures are pretreated for l-2 weeks with NGF in RPM1 1640 medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed and passaged into serum-free RPM1 1640 medium.
Animal Research Rats are randomly allocated into five groups: sham group (n=5), control group with IIR (n=8) and three groups with IIR who are given N-acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min before ischemia (n=8, group N-acetylcysteine 150), 300 mg/kg i.p 5 min before ischemia (n=7, group N-acetylcysteine 300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, group N-acetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from the abdominal aorta.[1]
Synonyms Reactive Oxygen Species, NAC, LNAC, Ferroptosis, Acetylcysteine, Influenza Virus, Inhibitor, Endogenous Metabolite, inhibit, N-Acetylcysteine, Apoptosis, N-Acetyl-L-cysteine
Molecular Weight 163.19
Formula C5H9NO3S
CAS No. 616-91-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 31 mg/mL (190 mM)

Ethanol: 31 mg/mL (190 mM)

H2O: 30 mg/mL (183.8 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Alvirez-Freites EJ, et al. Antimicrob Agents Chemother, 2002, 46(4), 1022-1025. 1. Ma X, Tan X, Yu B, et al. DOCK2 regulates antifungal immunity by regulating RAC GTPase activity. Cellular & Molecular Immunology. 2022: 1-17. 2. Tang Y, Song H, Wang Z, et al. Repurposing antiparasitic antimonials to noncovalently rescue temperature-sensitive p53 mutations. Cell Reports. 2022, 39(2): 110622 2. Ferrari G, et al. N-acetylcysteine (D- and L-stereoisomers) prevents apoptotic death of neuronal cells. J Neurosci. 1995 Apr;15(4):2857-66. 3. Tsai JC, et al. J Biol Chem, 1996, 271(7), 3667-3670. 3. Xu B, Qin Y, Li D, et al. Inhibition of PDE4 protects neurons against oxygen-glucose deprivation-induced endoplasmic reticulum stress through activation of the Nrf-2/HO-1 pathway. Redox Biology. 2020, 28: 101342 4. Wu X, Ren Y, Wen Y, et al. Deacetylation of ZKSCAN3 by SIRT1 induces autophagy and protects SN4741 cells against MPP+-induced oxidative stress. Free Radical Biology and Medicine. 2022 4. Yan CY, et al. Prevention of PC12 cell death by N-acetylcysteine requires activation of the Ras pathway. J Neurosci. 1998 Jun 1;18(11):4042-9. 5. Xue J, Liao Q, Luo M, et al. Cigarette smoke-induced oxidative stress activates NRF2 to mediate fibronectin disorganization in vascular formation. Open Biology. 2022, 12(4): 210310 5. Farr SA, et al. J Neurochem, 2003, 84(5), 1173-1183. 6. Yi Y, Gao K, Zhang L, et al. Zearalenone Induces MLKL-Dependent Necroptosis in Goat Endometrial Stromal Cells via the Calcium Overload/ROS Pathway. International Journal of Molecular Sciences. 2022, 23(17): 10170. 6. Kalimeris K, et al. N-acetylcysteine ameliorates liver injury in a rat model of intestinal ischemia reperfusion. J Surg Res. 2016 Dec;206(2):263-272. 7. Xu B, Xu J, Cai N, et al. Roflumilast prevents ischemic stroke-induced neuronal damage by restricting GSK3β-mediated oxidative stress and IRE1α/TRAF2/JNK pathway[J]. Free Radical Biology and Medicine. 2020 7. Xu B, Xu J, Cai N, et al. Roflumilast prevents ischemic stroke-induced neuronal damage by restricting GSK3β-mediated oxidative stress and IRE1α/TRAF2/JNK pathway. Free Radical Biology and Medicine. 2020 8. Zhang Y, Zhang C, Li J, et al. Inhibition of AKT induces p53/SIRT6/PARP1-dependent parthanatos to suppress tumor growth. Cell Communication and Signaling. 2022, 20(1): 1-21 8. Xu B, Qin Y, Li D, et al. Inhibition of PDE4 protects neurons against oxygen-glucose deprivation-induced endoplasmic reticulum stress through activation of the Nrf-2/HO-1 pathway[J]. Redox Biology. 2020, 28: 101342. 9. Zhu M, Tang X, Gong Z, et al. TAD1822-7 induces ROS-mediated apoptosis of HER2 positive breast cancer by decreasing E-cadherin in an EphB4 dependent manner. Life Sciences. 2021: 119954 10. Lin L, Chen Y, Li Q, et al. Isoxanthohumol, a component of Sophora flavescens, promotes the activation of the NLRP3 inflammasome and induces idiosyncratic hepatotoxicity. Journal of Ethnopharmacology. 2021: 114796. 11. Yi Y, Gao K, Lin P, et al. Staphylococcus aureus-Induced Necroptosis Promotes Mitochondrial Damage in Goat Endometrial Epithelial Cells. Animals. 2022, 12(17): 2218. 12. Zhang P, Zhang J, Quan H, et al. Effects of butein on human osteosarcoma cell proliferation, apoptosis, and autophagy through oxidative stress. Human & Experimental Toxicology. 2022, 41: 09603271221074346.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Compound Library HIF-1 Signaling Pathway Compound Library Traditional Chinese Medicine Monomer Library Human Endogenous Metabolite Library Anti-Obesity Compound Library Inhibitor Library Drug-induced Liver Injury (DILI) Compound Library Natural Product Library for HTS Anti-Tumor Natural Product Library Immuno-Oncology Compound Library

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